Patent
US 10,633,698A COMPOSITION FOR PCR CONTAINING A POLYETHYLENE GLYCOL-ENGRAFTED NANO-SIZED GRAPHENE OXIDE
Dong Eun KIM
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 10,633,698Patent drawings and their descriptions. Click a drawing to enlarge it.
FIGS. 1A-1 C includes schematic diagrams showing a process by which the efficiency and specificity of PCR may be increased by the polyethylene glycol-engrafted …
FIG. 2A, according to AFM analysis, the GO p ar ticles exhibited a br oad particle distribution of 400 to 1,000 nm, whereas the diameters of the nG O and P …
FIG. 3B is an image sh o wing the degree of PC R amplificati o n when PC R was perf o rmed o n a sample c o ntaining PEG-nG O (PEG and nG O were combined) o r a …
FIG. 4B is an image sh o wing the result f o r c o nfirming f o rmati o n of amplic ons depending o n the presence o r absence of P EG-nGO. P CR pr o ducts o …
FIG. 5 A is an image sh ow ing the result of DNA amplificati o n depending o n the presence o r absence of P EG-nG O in PC R samples to which va rio us …
FIG. 6 is an image showing the result f o r confirming the effect of PEG-nG O o n the efficiency and specificity of PCR depending on annealing temperature;
FIG. 7B. As the co ncentrati o n of the P EG-nG O increased, Tm decreased. These results indicate that the P EG-nG O may promote dissociation of dsDNA. <6 -3> …
FIG. 8 D is an image showing the result of performing PCR o n a PCR sample in which linear pET22b pl a smid DNA was used as a template and P EG-nG O was …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A composition for polymerase chain reaction (PCR), comprising 6-arm polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO), wherein the PEG-nGO has a thickness of 4 to nanometers (nm). Currently amended
The composition for PCR according to claim 1, wherein the PEG-nGO is present at a concentration of 1 to 10 p g/ml. Original
The composition for PCR according to claim 1, wherein each of a forward primer and a reverse primer is added to the composition for PCR at a concentration of 0.1 to 1.0 M. Original
The composition for PCR according to claim 1, wherein the PEG-nGO increases efficiency and specificity of PCR by inhibiting primer dimerization and nonspecific binding of amplified amplicons. Original
The composition for PCR according to claim 1, wherein the PEG-nGO promotes denaturation of double-stranded DNA in a sample to shorten PCR time. Original
A polymerase chain reaction (PCR) kit, comprising the composition according to claim 1. Original
A polymerase chain reaction (PCR) method, comprising: preparing a PCR sample by mixing a DNA template, dNTPs, a DNA polymerase, and forward and reverse primers for amplifying a target sequence with the composition for PCR according to claim 1; and performing PCR using the prepared PCR sample. Original
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials
Atomic force microscopy (AFM) analysis was performed to confirm particle sizes of GO, nGO, and PEG-nGO. FT-IR analysis was also performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO particles.
3 materials1 process step
PCR was performed on samples containing GO, nGO, or PEG-nGO at various concentrations to confirm the degree of PCR amplification. The binding affinity of GO, nGO, and PEG-nGO to ssDNA and Taq DNA polymerase was also confirmed. PEG-nGO (PEG covalently combined with nGO) was compared with a simple mixture of PEG + nGO.
1 material1 process step
The optimal concentration of PEG-nGO was confirmed when added to a PCR sample. PCR products from the first round were diluted stepwise and the second round of PCR was performed with or without PEG-nGO. Five consecutive rounds of PCR were also performed with or without PEG-nGO.
Measurements and analyses referenced in the patent, with their drawing references.
AFM analysis performed to confirm particle sizes of GO, nGO, and PEG-nGO
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
PEG-nGO thickness (claimed range: 4 to [unspecified] nm) | ≥ 4 nm | 6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
PEG-nGO concentration in PCR composition |
Patent
Atlas literature
Patent
US 10,633,698Patent drawings and their descriptions. Click a drawing to enlarge it.
FIGS. 1A-1 C includes schematic diagrams showing a process by which the efficiency and specificity of PCR may be increased by the polyethylene glycol-engrafted …
FIG. 2A, according to AFM analysis, the GO p ar ticles exhibited a br oad particle distribution of 400 to 1,000 nm, whereas the diameters of the nG O and P …
FIG. 3B is an image sh o wing the degree of PC R amplificati o n when PC R was perf o rmed o n a sample c o ntaining PEG-nG O (PEG and nG O were combined) o r a …
FIG. 4B is an image sh o wing the result f o r c o nfirming f o rmati o n of amplic ons depending o n the presence o r absence of P EG-nGO. P CR pr o ducts o …
FIG. 5 A is an image sh ow ing the result of DNA amplificati o n depending o n the presence o r absence of P EG-nG O in PC R samples to which va rio us …
FIG. 6 is an image showing the result f o r confirming the effect of PEG-nG O o n the efficiency and specificity of PCR depending on annealing temperature;
FIG. 7B. As the co ncentrati o n of the P EG-nG O increased, Tm decreased. These results indicate that the P EG-nG O may promote dissociation of dsDNA. <6 -3> …
FIG. 8 D is an image showing the result of performing PCR o n a PCR sample in which linear pET22b pl a smid DNA was used as a template and P EG-nG O was …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A composition for polymerase chain reaction (PCR), comprising 6-arm polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO), wherein the PEG-nGO has a thickness of 4 to nanometers (nm). Currently amended
The composition for PCR according to claim 1, wherein the PEG-nGO is present at a concentration of 1 to 10 p g/ml. Original
The composition for PCR according to claim 1, wherein each of a forward primer and a reverse primer is added to the composition for PCR at a concentration of 0.1 to 1.0 M. Original
The composition for PCR according to claim 1, wherein the PEG-nGO increases efficiency and specificity of PCR by inhibiting primer dimerization and nonspecific binding of amplified amplicons. Original
The composition for PCR according to claim 1, wherein the PEG-nGO promotes denaturation of double-stranded DNA in a sample to shorten PCR time. Original
A polymerase chain reaction (PCR) kit, comprising the composition according to claim 1. Original
A polymerase chain reaction (PCR) method, comprising: preparing a PCR sample by mixing a DNA template, dNTPs, a DNA polymerase, and forward and reverse primers for amplifying a target sequence with the composition for PCR according to claim 1; and performing PCR using the prepared PCR sample. Original
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials
Atomic force microscopy (AFM) analysis was performed to confirm particle sizes of GO, nGO, and PEG-nGO. FT-IR analysis was also performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO particles.
3 materials1 process step
PCR was performed on samples containing GO, nGO, or PEG-nGO at various concentrations to confirm the degree of PCR amplification. The binding affinity of GO, nGO, and PEG-nGO to ssDNA and Taq DNA polymerase was also confirmed. PEG-nGO (PEG covalently combined with nGO) was compared with a simple mixture of PEG + nGO.
1 material1 process step
The optimal concentration of PEG-nGO was confirmed when added to a PCR sample. PCR products from the first round were diluted stepwise and the second round of PCR was performed with or without PEG-nGO. Five consecutive rounds of PCR were also performed with or without PEG-nGO.
Measurements and analyses referenced in the patent, with their drawing references.
AFM analysis performed to confirm particle sizes of GO, nGO, and PEG-nGO
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
PEG-nGO thickness (claimed range: 4 to [unspecified] nm) | ≥ 4 nm | 6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
PEG-nGO concentration in PCR composition |
Patent
Atlas literature
Patent
US 10,633,698Patent drawings and their descriptions. Click a drawing to enlarge it.
FIGS. 1A-1 C includes schematic diagrams showing a process by which the efficiency and specificity of PCR may be increased by the polyethylene glycol-engrafted …
FIG. 2A, according to AFM analysis, the GO p ar ticles exhibited a br oad particle distribution of 400 to 1,000 nm, whereas the diameters of the nG O and P …
FIG. 3B is an image sh o wing the degree of PC R amplificati o n when PC R was perf o rmed o n a sample c o ntaining PEG-nG O (PEG and nG O were combined) o r a …
FIG. 4B is an image sh o wing the result f o r c o nfirming f o rmati o n of amplic ons depending o n the presence o r absence of P EG-nGO. P CR pr o ducts o …
FIG. 5 A is an image sh ow ing the result of DNA amplificati o n depending o n the presence o r absence of P EG-nG O in PC R samples to which va rio us …
FIG. 6 is an image showing the result f o r confirming the effect of PEG-nG O o n the efficiency and specificity of PCR depending on annealing temperature;
FIG. 7B. As the co ncentrati o n of the P EG-nG O increased, Tm decreased. These results indicate that the P EG-nG O may promote dissociation of dsDNA. <6 -3> …
FIG. 8 D is an image showing the result of performing PCR o n a PCR sample in which linear pET22b pl a smid DNA was used as a template and P EG-nG O was …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A composition for polymerase chain reaction (PCR), comprising 6-arm polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO), wherein the PEG-nGO has a thickness of 4 to nanometers (nm). Currently amended
The composition for PCR according to claim 1, wherein the PEG-nGO is present at a concentration of 1 to 10 p g/ml. Original
The composition for PCR according to claim 1, wherein each of a forward primer and a reverse primer is added to the composition for PCR at a concentration of 0.1 to 1.0 M. Original
The composition for PCR according to claim 1, wherein the PEG-nGO increases efficiency and specificity of PCR by inhibiting primer dimerization and nonspecific binding of amplified amplicons. Original
The composition for PCR according to claim 1, wherein the PEG-nGO promotes denaturation of double-stranded DNA in a sample to shorten PCR time. Original
A polymerase chain reaction (PCR) kit, comprising the composition according to claim 1. Original
A polymerase chain reaction (PCR) method, comprising: preparing a PCR sample by mixing a DNA template, dNTPs, a DNA polymerase, and forward and reverse primers for amplifying a target sequence with the composition for PCR according to claim 1; and performing PCR using the prepared PCR sample. Original
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials
Atomic force microscopy (AFM) analysis was performed to confirm particle sizes of GO, nGO, and PEG-nGO. FT-IR analysis was also performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO particles.
3 materials1 process step
PCR was performed on samples containing GO, nGO, or PEG-nGO at various concentrations to confirm the degree of PCR amplification. The binding affinity of GO, nGO, and PEG-nGO to ssDNA and Taq DNA polymerase was also confirmed. PEG-nGO (PEG covalently combined with nGO) was compared with a simple mixture of PEG + nGO.
1 material1 process step
The optimal concentration of PEG-nGO was confirmed when added to a PCR sample. PCR products from the first round were diluted stepwise and the second round of PCR was performed with or without PEG-nGO. Five consecutive rounds of PCR were also performed with or without PEG-nGO.
Measurements and analyses referenced in the patent, with their drawing references.
AFM analysis performed to confirm particle sizes of GO, nGO, and PEG-nGO
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
PEG-nGO thickness (claimed range: 4 to [unspecified] nm) | ≥ 4 nm | 6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
PEG-nGO concentration in PCR composition |
Patent
Atlas literature
Patent
US 10,633,698Patent drawings and their descriptions. Click a drawing to enlarge it.
FIGS. 1A-1 C includes schematic diagrams showing a process by which the efficiency and specificity of PCR may be increased by the polyethylene glycol-engrafted …
FIG. 2A, according to AFM analysis, the GO p ar ticles exhibited a br oad particle distribution of 400 to 1,000 nm, whereas the diameters of the nG O and P …
FIG. 3B is an image sh o wing the degree of PC R amplificati o n when PC R was perf o rmed o n a sample c o ntaining PEG-nG O (PEG and nG O were combined) o r a …
FIG. 4B is an image sh o wing the result f o r c o nfirming f o rmati o n of amplic ons depending o n the presence o r absence of P EG-nGO. P CR pr o ducts o …
FIG. 5 A is an image sh ow ing the result of DNA amplificati o n depending o n the presence o r absence of P EG-nG O in PC R samples to which va rio us …
FIG. 6 is an image showing the result f o r confirming the effect of PEG-nG O o n the efficiency and specificity of PCR depending on annealing temperature;
FIG. 7B. As the co ncentrati o n of the P EG-nG O increased, Tm decreased. These results indicate that the P EG-nG O may promote dissociation of dsDNA. <6 -3> …
FIG. 8 D is an image showing the result of performing PCR o n a PCR sample in which linear pET22b pl a smid DNA was used as a template and P EG-nG O was …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A composition for polymerase chain reaction (PCR), comprising 6-arm polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO), wherein the PEG-nGO has a thickness of 4 to nanometers (nm). Currently amended
The composition for PCR according to claim 1, wherein the PEG-nGO is present at a concentration of 1 to 10 p g/ml. Original
The composition for PCR according to claim 1, wherein each of a forward primer and a reverse primer is added to the composition for PCR at a concentration of 0.1 to 1.0 M. Original
The composition for PCR according to claim 1, wherein the PEG-nGO increases efficiency and specificity of PCR by inhibiting primer dimerization and nonspecific binding of amplified amplicons. Original
The composition for PCR according to claim 1, wherein the PEG-nGO promotes denaturation of double-stranded DNA in a sample to shorten PCR time. Original
A polymerase chain reaction (PCR) kit, comprising the composition according to claim 1. Original
A polymerase chain reaction (PCR) method, comprising: preparing a PCR sample by mixing a DNA template, dNTPs, a DNA polymerase, and forward and reverse primers for amplifying a target sequence with the composition for PCR according to claim 1; and performing PCR using the prepared PCR sample. Original
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials
Atomic force microscopy (AFM) analysis was performed to confirm particle sizes of GO, nGO, and PEG-nGO. FT-IR analysis was also performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO particles.
3 materials1 process step
PCR was performed on samples containing GO, nGO, or PEG-nGO at various concentrations to confirm the degree of PCR amplification. The binding affinity of GO, nGO, and PEG-nGO to ssDNA and Taq DNA polymerase was also confirmed. PEG-nGO (PEG covalently combined with nGO) was compared with a simple mixture of PEG + nGO.
1 material1 process step
The optimal concentration of PEG-nGO was confirmed when added to a PCR sample. PCR products from the first round were diluted stepwise and the second round of PCR was performed with or without PEG-nGO. Five consecutive rounds of PCR were also performed with or without PEG-nGO.
Measurements and analyses referenced in the patent, with their drawing references.
AFM analysis performed to confirm particle sizes of GO, nGO, and PEG-nGO
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
PEG-nGO thickness (claimed range: 4 to [unspecified] nm) | ≥ 4 nm | 6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
PEG-nGO concentration in PCR composition |
FT-IR analysis performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO
Gel electrophoresis used to confirm degree of PCR amplification and specificity when GO, nGO, or PEG-nGO was added to PCR samples
6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
Thickness | 400–1000 nm | — |
Thickness | 1.2–1.6 nm | — |
Thickness | 4–5 nm | — |
FT-IR analysis performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO
Gel electrophoresis used to confirm degree of PCR amplification and specificity when GO, nGO, or PEG-nGO was added to PCR samples
6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
Thickness | 400–1000 nm | — |
Thickness | 1.2–1.6 nm | — |
Thickness | 4–5 nm | — |
FT-IR analysis performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO
Gel electrophoresis used to confirm degree of PCR amplification and specificity when GO, nGO, or PEG-nGO was added to PCR samples
6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
Thickness | 400–1000 nm | — |
Thickness | 1.2–1.6 nm | — |
Thickness | 4–5 nm | — |
FT-IR analysis performed to confirm characteristic functional groups of GO, nGO, and PEG-nGO
Gel electrophoresis used to confirm degree of PCR amplification and specificity when GO, nGO, or PEG-nGO was added to PCR samples
6-arm polyethylene glycol-engrafted nano-sized graphene oxide |
Thickness | 400–1000 nm | — |
Thickness | 1.2–1.6 nm | — |
Thickness | 4–5 nm | — |