"Graphene Hydrogel and Methods for Using the Same," ﬁled on February 23, 5 2013, by

Subhra Mohapatra

Patent

Atlas literature

Patent

US 9,434,926

"Graphene Hydrogel and Methods for Using the Same," ﬁled on February 23, 5 2013, by

Subhra Mohapatra

Drawings

Patent drawings and their descriptions. Click a drawing to enlarge it.

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Claims

Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.

1 independent · 20 dependent

1
Independentgraphenechitosancarboxylic acid functionalized graphenepolyethylene glycol diacrylate (PEGDA)graphene hydrogel
A method comprising: contacting a cell with a hydrogel for a period of time, where the hydrogel comprises: graphene, a chitosan, and a polyethylene (glycol) diacrylate (PEGDA) at least two acrylated chitosan-qraphene monomers, where each chitosan-qraphene monomer comprises: carboxylic acid functionalized qraphene, where the chitosan component of the chitosan-qraphene monomer is covalently bonded to the carboxylic acid functionalized qraphene component of the chitosan-qraphene monomer; and pol y ethylene (qlycol) diacrylate (PEGDA), where the (PEGDA) covalently crosslinks the at least two acrylated chitosan-qraphene monomers.
1. 2
  Dependent← claim 1
  The method of claim 1, further comprising the step of culturing the cell and the hydrogel in cell culture media.
2. 6
  Dependent← claim 1
  The method of claim 1, wherein the cell is a stem cell.
3. 8
  Dependent← claim 1
  The method of claim 1, wherein the cell is a bone-marrow mesenchymal stem cell.
4. 9
  Dependent← claim 1
  The method of claim 1, where the period of time ranges from about 1 hour to about 14 days.
5. 10
  Dependent← claim 1
  The method of claim 1, wherein the cell proliferates to form a population of cells after the period of time. -3- 4818-3041-5917, v 1
6. 14
  Dependent← claim 1
  The method claim 1, wherein after the period of time the cell diﬀerentiates to form a diﬀerentiated population of cells.
7. 21
  Dependent← claim 1N-Isopropylacrylamide (NIPAM)
  The method of claim 1, wherein the hydrogel further comprises N-Isopropylacrylamide (NIPAM), where the NIPAM is crosslinked at least one of the acrylated chitosan-graphenemonomers. -5- 4818-3041-5917, v 1

Device structures

Layer stacks claimed or described, ordered top of device to substrate.

graphene hydrogel

polyethylene glycol diacrylate (PEGDA)crosslinker

chitosanmatrix component

graphenematrix component

Materials

Materials described outside the worked examples.

graphene

Hydrogel Component

chitosan

Hydrogel Component

Reported properties

Performance values and ranges asserted in the specification or claims.

Property	Value	Material
Temperature	12–14 k	—
Thickness

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