Patent
US 10,788,486GRAPHENE OXIDE-BASED NANOLAB AND METHODS OF DETECTING OF EXOSOMES
Peng Zhang, Yong Zeng, Mei He
US
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Patent
Atlas literature
Patent
US 10,788,486Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A graphene-oxide coated particle for capturing a biological substance, the particle comprising: a core particle having a surface; a graphene-oxide-layer coating said core particle, said graphene-oxide la y er comprising graphene-oxide nanosheets, said graphene-oxide nanosheets being covalently bonded to the particl surface; at least one polydopamine polymer coupled with the graphene-oxide nanosheets; and at least one targeting receptor coupled to the at least one polydopamine polymer, wherein the targeting receptor is capable of targeting and binding with a target biological substance. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle includes a functional group covalently coupled with a functional group of the graphene-oxide layer to form the covalent bond between the surface and graphene-oxide nanosheets. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets include a functional group that is covalently coupled with a functional group of the at least one polydopamine polymer to form a covalent bond between the graphene-oxide nanosheets and polydopamine polymer. Currently amended
The graphene-oxide coated particle of claim 1, wherein the at least one polydopamine polymer includes a functional group that is covalently coupled with a functional group of the at least one targeting receptor to form a covalent bond between the polydopamine polymer and targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, wherein the targeting receptor is an antibody or fragment thereof. Currently amended
The graphene-oxide coated particle of claim 1, wherein the target biological substance is an exosome. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle is a core/shell magnetic particle having a magnetic core and a functionalized shell. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets are covalently bonded to the at least one polydopamine polymer; and wherein the at least one polydopamine polymer is covalently bonded to the at least one targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, comprising the target biological substance bound to the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of capturing a target biological substance, the method comprising: providing the graphene-oxide coated particle of claim 1; and contacting a sample with the graphene-oxide coated particle such that the target biological substance associates with the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of determining a disease state in a subject, the method comprising: providing the graphene-oxide coated particle of claim 1; obtaining a sample from the subject; contacting the sample with the graphene-oxide coated particle; and detecting the presence of the target biological substance in the sample, wherein if the target biological substance binds with the targeting receptor, the target biological substance is present in the sample, or if the target biological substance does not bind with the targeting receptor, the target biological substance is absent in the sample to thereby determine the disease state in the subject s-,. Currently amended
The graphene-oxide coated particle of claim 1, wherein said particle is a magnetic bead. Currently amended
The graphene-oxide coated particle of claim 1, wherein said graphene-oxide layer comprises a plurality of said graphene-oxide nanosheets covalently bonded to said substrate surface, said graphene-oxide nanosheets having a plurality of Currently amended
-9.. Canceled
Canceled
. Canceled
. Canceled
The graphene-oxide coated particle of claim from the graphene-oxide layer away from the
. Canceled
The graphene-oxide coated particle of claim polymers form a layer having a 3-dimensional, New polydopamine polymers coupled thereto. 22, wherein each polydopamine polymer extends particle. Currently amended 22, wherein said plurality of polydopamine porous morphology on said graphene-oxide layer.
Layer stacks claimed or described, ordered top of device to substrate.
graphene-oxide coated particle capture device
microfluidic chip with Y-shaped microposts coated with GO-PDA
Materials described outside the worked examples.
graphene oxide nanosheets
polydopamine polymer
Patent
Atlas literature
Patent
US 10,788,486Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A graphene-oxide coated particle for capturing a biological substance, the particle comprising: a core particle having a surface; a graphene-oxide-layer coating said core particle, said graphene-oxide la y er comprising graphene-oxide nanosheets, said graphene-oxide nanosheets being covalently bonded to the particl surface; at least one polydopamine polymer coupled with the graphene-oxide nanosheets; and at least one targeting receptor coupled to the at least one polydopamine polymer, wherein the targeting receptor is capable of targeting and binding with a target biological substance. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle includes a functional group covalently coupled with a functional group of the graphene-oxide layer to form the covalent bond between the surface and graphene-oxide nanosheets. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets include a functional group that is covalently coupled with a functional group of the at least one polydopamine polymer to form a covalent bond between the graphene-oxide nanosheets and polydopamine polymer. Currently amended
The graphene-oxide coated particle of claim 1, wherein the at least one polydopamine polymer includes a functional group that is covalently coupled with a functional group of the at least one targeting receptor to form a covalent bond between the polydopamine polymer and targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, wherein the targeting receptor is an antibody or fragment thereof. Currently amended
The graphene-oxide coated particle of claim 1, wherein the target biological substance is an exosome. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle is a core/shell magnetic particle having a magnetic core and a functionalized shell. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets are covalently bonded to the at least one polydopamine polymer; and wherein the at least one polydopamine polymer is covalently bonded to the at least one targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, comprising the target biological substance bound to the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of capturing a target biological substance, the method comprising: providing the graphene-oxide coated particle of claim 1; and contacting a sample with the graphene-oxide coated particle such that the target biological substance associates with the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of determining a disease state in a subject, the method comprising: providing the graphene-oxide coated particle of claim 1; obtaining a sample from the subject; contacting the sample with the graphene-oxide coated particle; and detecting the presence of the target biological substance in the sample, wherein if the target biological substance binds with the targeting receptor, the target biological substance is present in the sample, or if the target biological substance does not bind with the targeting receptor, the target biological substance is absent in the sample to thereby determine the disease state in the subject s-,. Currently amended
The graphene-oxide coated particle of claim 1, wherein said particle is a magnetic bead. Currently amended
The graphene-oxide coated particle of claim 1, wherein said graphene-oxide layer comprises a plurality of said graphene-oxide nanosheets covalently bonded to said substrate surface, said graphene-oxide nanosheets having a plurality of Currently amended
-9.. Canceled
Canceled
. Canceled
. Canceled
The graphene-oxide coated particle of claim from the graphene-oxide layer away from the
. Canceled
The graphene-oxide coated particle of claim polymers form a layer having a 3-dimensional, New polydopamine polymers coupled thereto. 22, wherein each polydopamine polymer extends particle. Currently amended 22, wherein said plurality of polydopamine porous morphology on said graphene-oxide layer.
Layer stacks claimed or described, ordered top of device to substrate.
graphene-oxide coated particle capture device
microfluidic chip with Y-shaped microposts coated with GO-PDA
Materials described outside the worked examples.
graphene oxide nanosheets
polydopamine polymer
Patent
Atlas literature
Patent
US 10,788,486Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A graphene-oxide coated particle for capturing a biological substance, the particle comprising: a core particle having a surface; a graphene-oxide-layer coating said core particle, said graphene-oxide la y er comprising graphene-oxide nanosheets, said graphene-oxide nanosheets being covalently bonded to the particl surface; at least one polydopamine polymer coupled with the graphene-oxide nanosheets; and at least one targeting receptor coupled to the at least one polydopamine polymer, wherein the targeting receptor is capable of targeting and binding with a target biological substance. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle includes a functional group covalently coupled with a functional group of the graphene-oxide layer to form the covalent bond between the surface and graphene-oxide nanosheets. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets include a functional group that is covalently coupled with a functional group of the at least one polydopamine polymer to form a covalent bond between the graphene-oxide nanosheets and polydopamine polymer. Currently amended
The graphene-oxide coated particle of claim 1, wherein the at least one polydopamine polymer includes a functional group that is covalently coupled with a functional group of the at least one targeting receptor to form a covalent bond between the polydopamine polymer and targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, wherein the targeting receptor is an antibody or fragment thereof. Currently amended
The graphene-oxide coated particle of claim 1, wherein the target biological substance is an exosome. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle is a core/shell magnetic particle having a magnetic core and a functionalized shell. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets are covalently bonded to the at least one polydopamine polymer; and wherein the at least one polydopamine polymer is covalently bonded to the at least one targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, comprising the target biological substance bound to the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of capturing a target biological substance, the method comprising: providing the graphene-oxide coated particle of claim 1; and contacting a sample with the graphene-oxide coated particle such that the target biological substance associates with the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of determining a disease state in a subject, the method comprising: providing the graphene-oxide coated particle of claim 1; obtaining a sample from the subject; contacting the sample with the graphene-oxide coated particle; and detecting the presence of the target biological substance in the sample, wherein if the target biological substance binds with the targeting receptor, the target biological substance is present in the sample, or if the target biological substance does not bind with the targeting receptor, the target biological substance is absent in the sample to thereby determine the disease state in the subject s-,. Currently amended
The graphene-oxide coated particle of claim 1, wherein said particle is a magnetic bead. Currently amended
The graphene-oxide coated particle of claim 1, wherein said graphene-oxide layer comprises a plurality of said graphene-oxide nanosheets covalently bonded to said substrate surface, said graphene-oxide nanosheets having a plurality of Currently amended
-9.. Canceled
Canceled
. Canceled
. Canceled
The graphene-oxide coated particle of claim from the graphene-oxide layer away from the
. Canceled
The graphene-oxide coated particle of claim polymers form a layer having a 3-dimensional, New polydopamine polymers coupled thereto. 22, wherein each polydopamine polymer extends particle. Currently amended 22, wherein said plurality of polydopamine porous morphology on said graphene-oxide layer.
Layer stacks claimed or described, ordered top of device to substrate.
graphene-oxide coated particle capture device
microfluidic chip with Y-shaped microposts coated with GO-PDA
Materials described outside the worked examples.
graphene oxide nanosheets
polydopamine polymer
Patent
Atlas literature
Patent
US 10,788,486Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A graphene-oxide coated particle for capturing a biological substance, the particle comprising: a core particle having a surface; a graphene-oxide-layer coating said core particle, said graphene-oxide la y er comprising graphene-oxide nanosheets, said graphene-oxide nanosheets being covalently bonded to the particl surface; at least one polydopamine polymer coupled with the graphene-oxide nanosheets; and at least one targeting receptor coupled to the at least one polydopamine polymer, wherein the targeting receptor is capable of targeting and binding with a target biological substance. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle includes a functional group covalently coupled with a functional group of the graphene-oxide layer to form the covalent bond between the surface and graphene-oxide nanosheets. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets include a functional group that is covalently coupled with a functional group of the at least one polydopamine polymer to form a covalent bond between the graphene-oxide nanosheets and polydopamine polymer. Currently amended
The graphene-oxide coated particle of claim 1, wherein the at least one polydopamine polymer includes a functional group that is covalently coupled with a functional group of the at least one targeting receptor to form a covalent bond between the polydopamine polymer and targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, wherein the targeting receptor is an antibody or fragment thereof. Currently amended
The graphene-oxide coated particle of claim 1, wherein the target biological substance is an exosome. Currently amended
The graphene-oxide coated particle of claim 1, wherein the core particle is a core/shell magnetic particle having a magnetic core and a functionalized shell. Currently amended
The graphene-oxide coated particle of claim 1, wherein the graphene-oxide nanosheets are covalently bonded to the at least one polydopamine polymer; and wherein the at least one polydopamine polymer is covalently bonded to the at least one targeting receptor. Currently amended
The graphene-oxide coated particle of claim 1, comprising the target biological substance bound to the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of capturing a target biological substance, the method comprising: providing the graphene-oxide coated particle of claim 1; and contacting a sample with the graphene-oxide coated particle such that the target biological substance associates with the targeting receptor. Currently amended
(Withdrawn-Currently Amended) A method of determining a disease state in a subject, the method comprising: providing the graphene-oxide coated particle of claim 1; obtaining a sample from the subject; contacting the sample with the graphene-oxide coated particle; and detecting the presence of the target biological substance in the sample, wherein if the target biological substance binds with the targeting receptor, the target biological substance is present in the sample, or if the target biological substance does not bind with the targeting receptor, the target biological substance is absent in the sample to thereby determine the disease state in the subject s-,. Currently amended
The graphene-oxide coated particle of claim 1, wherein said particle is a magnetic bead. Currently amended
The graphene-oxide coated particle of claim 1, wherein said graphene-oxide layer comprises a plurality of said graphene-oxide nanosheets covalently bonded to said substrate surface, said graphene-oxide nanosheets having a plurality of Currently amended
-9.. Canceled
Canceled
. Canceled
. Canceled
The graphene-oxide coated particle of claim from the graphene-oxide layer away from the
. Canceled
The graphene-oxide coated particle of claim polymers form a layer having a 3-dimensional, New polydopamine polymers coupled thereto. 22, wherein each polydopamine polymer extends particle. Currently amended 22, wherein said plurality of polydopamine porous morphology on said graphene-oxide layer.
Layer stacks claimed or described, ordered top of device to substrate.
graphene-oxide coated particle capture device
microfluidic chip with Y-shaped microposts coated with GO-PDA
Materials described outside the worked examples.
graphene oxide nanosheets
polydopamine polymer
targeting receptor
magnetic core/shell particle
anti-CD₈₁ monoclonal antibody
exosome
targeting receptor
magnetic core/shell particle
anti-CD₈₁ monoclonal antibody
exosome
targeting receptor
magnetic core/shell particle
anti-CD₈₁ monoclonal antibody
exosome
targeting receptor
magnetic core/shell particle
anti-CD₈₁ monoclonal antibody
exosome