Patent
US 10,138,126SIMPLE PRODUCTION METHOD FOR GRAPHENE BY MICROORGANISMS
Mariam Al Ali Alamaadeed
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 10,138,126Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 shows an exemplary process for producing graphene from GO according to one embodiment of the invention. [00010] FIGs. 2(a)-(b) are scanning electron …
FIGS. 2-6. SEM images may be aggregated, thin, crumpled sheets closely associated with each other and forming a disordered solid as illustrated in in
FIG. 7(a) is an X-Ray diffraction ("XRD") graph of graphite. [00016]
FIG. 8 is an XRD graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00018]
FIG. 9 is an XRD graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00019]
FIG. 10 is an XRD graph of graphene prepared with Can d ida a lb ica ns according to one embodiment of the invention. [00020]
FIG. 11 is an XRD graph of graphene prepared with Streptococcus fiaecalis according to one embodiment of the invention. [00021]
FIG. 12 is an XRD graph of graphene prepared with Staphylococcus aureus according to one embodiment of the invention. [00022]
FIG. 13(b) is a Raman Spectroscopy graph of graphene oxide. [00024]
FIG. 14 is a Raman Spectroscopy graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00025]
FIG. 15 is a Raman Spectroscopy graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00026]
FIG. 16 is a Raman Spectroscopy graph of graphene prepared with Cand ida albica n s according to one embodiment of the invention. [00027]
FIG. 17 is a Raman Spectroscopy graph of graphene prepared with Streptococcus faecali s according to one embodiment of the invention. [00028]
FIG. 18 is a Raman Spectroscopy graph of graphene prepared with Staphylococcus a ureu s according to one embodiment of the invention.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of aerobically producing graphene from graphene oxide with a microorganism comprising: a) g rowing one or more microorganism cultures in suitable media under aerobic conditions; b) reducing concentration of the one or more cultures between approximately 10 2 CFU/mL to approximately 1 01 0 CFU/mL, wherein said reduced concentration comprises transferring a portion of the culture to a vessel; c) adding graphene oxide to the vessel under atmospheric conditions; d) removing the added graphene oxide from the vessel after a predetermined time, wherein said removed graphene oxide contains graphene; and e) washing the removed graphene oxide with a solution to recover the graphene, wherein the y ield of recovered graphene is greater than 50 % of the added graphene oxide; wherein the one or more microorganism cultures is selected from the group consisting of: Pseudomonas aeruginosa, Candida albicans, Streptococcus faecalis, Staphylococcus aureus, algae, cyanobacteria, and combinations thereof, wherein the microorganism reduces graphene oxide to graphene. Currently amended
The method of claim 1, wherein the suitable media is nutrient broth. Original
The method of claim 1, wherein the portion is approximately 10 1. Original
The method of claim 1, wherein the graphene oxide is a film of graphene oxide at a concentration of approximately 30mg per 10 4 CFU/mL of the one or more cultures. Previously presented
The method of claim 1, wherein the aerobic conditions comprise a period of 24 hours, a temperature from approximately 25 ° C to approximately 40 ° C, absence of light or a shaker without any additional gas. Original
The method of claim 1, wherein the concentration of the one or more cultures is approximately 1 0 4 CFU/mL. Original
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than 90/of the added graphene oxide. Currently amended
The method o claim 1, where the solution [[is]] in step e) comprises saline. Currently amended
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added in the absence of light. Withdrawn
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added under vacuum pressure. Withdrawn
The method of claim 1, wherein the predetermined
The method of claim 1, wherein the predetermined time is approximately 2-20 days. Original time is approximately 20 days. Original
Canceled
Canceled
Canceled
Canceled
Materials described outside the worked examples.
graphene oxide
graphene
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
graphite basal reflection (002) | 26.56 deg | natural graphite flakes |
graphene oxide diffraction peak |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,138,126Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 shows an exemplary process for producing graphene from GO according to one embodiment of the invention. [00010] FIGs. 2(a)-(b) are scanning electron …
FIGS. 2-6. SEM images may be aggregated, thin, crumpled sheets closely associated with each other and forming a disordered solid as illustrated in in
FIG. 7(a) is an X-Ray diffraction ("XRD") graph of graphite. [00016]
FIG. 8 is an XRD graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00018]
FIG. 9 is an XRD graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00019]
FIG. 10 is an XRD graph of graphene prepared with Can d ida a lb ica ns according to one embodiment of the invention. [00020]
FIG. 11 is an XRD graph of graphene prepared with Streptococcus fiaecalis according to one embodiment of the invention. [00021]
FIG. 12 is an XRD graph of graphene prepared with Staphylococcus aureus according to one embodiment of the invention. [00022]
FIG. 13(b) is a Raman Spectroscopy graph of graphene oxide. [00024]
FIG. 14 is a Raman Spectroscopy graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00025]
FIG. 15 is a Raman Spectroscopy graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00026]
FIG. 16 is a Raman Spectroscopy graph of graphene prepared with Cand ida albica n s according to one embodiment of the invention. [00027]
FIG. 17 is a Raman Spectroscopy graph of graphene prepared with Streptococcus faecali s according to one embodiment of the invention. [00028]
FIG. 18 is a Raman Spectroscopy graph of graphene prepared with Staphylococcus a ureu s according to one embodiment of the invention.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of aerobically producing graphene from graphene oxide with a microorganism comprising: a) g rowing one or more microorganism cultures in suitable media under aerobic conditions; b) reducing concentration of the one or more cultures between approximately 10 2 CFU/mL to approximately 1 01 0 CFU/mL, wherein said reduced concentration comprises transferring a portion of the culture to a vessel; c) adding graphene oxide to the vessel under atmospheric conditions; d) removing the added graphene oxide from the vessel after a predetermined time, wherein said removed graphene oxide contains graphene; and e) washing the removed graphene oxide with a solution to recover the graphene, wherein the y ield of recovered graphene is greater than 50 % of the added graphene oxide; wherein the one or more microorganism cultures is selected from the group consisting of: Pseudomonas aeruginosa, Candida albicans, Streptococcus faecalis, Staphylococcus aureus, algae, cyanobacteria, and combinations thereof, wherein the microorganism reduces graphene oxide to graphene. Currently amended
The method of claim 1, wherein the suitable media is nutrient broth. Original
The method of claim 1, wherein the portion is approximately 10 1. Original
The method of claim 1, wherein the graphene oxide is a film of graphene oxide at a concentration of approximately 30mg per 10 4 CFU/mL of the one or more cultures. Previously presented
The method of claim 1, wherein the aerobic conditions comprise a period of 24 hours, a temperature from approximately 25 ° C to approximately 40 ° C, absence of light or a shaker without any additional gas. Original
The method of claim 1, wherein the concentration of the one or more cultures is approximately 1 0 4 CFU/mL. Original
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than 90/of the added graphene oxide. Currently amended
The method o claim 1, where the solution [[is]] in step e) comprises saline. Currently amended
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added in the absence of light. Withdrawn
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added under vacuum pressure. Withdrawn
The method of claim 1, wherein the predetermined
The method of claim 1, wherein the predetermined time is approximately 2-20 days. Original time is approximately 20 days. Original
Canceled
Canceled
Canceled
Canceled
Materials described outside the worked examples.
graphene oxide
graphene
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
graphite basal reflection (002) | 26.56 deg | natural graphite flakes |
graphene oxide diffraction peak |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,138,126Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 shows an exemplary process for producing graphene from GO according to one embodiment of the invention. [00010] FIGs. 2(a)-(b) are scanning electron …
FIGS. 2-6. SEM images may be aggregated, thin, crumpled sheets closely associated with each other and forming a disordered solid as illustrated in in
FIG. 7(a) is an X-Ray diffraction ("XRD") graph of graphite. [00016]
FIG. 8 is an XRD graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00018]
FIG. 9 is an XRD graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00019]
FIG. 10 is an XRD graph of graphene prepared with Can d ida a lb ica ns according to one embodiment of the invention. [00020]
FIG. 11 is an XRD graph of graphene prepared with Streptococcus fiaecalis according to one embodiment of the invention. [00021]
FIG. 12 is an XRD graph of graphene prepared with Staphylococcus aureus according to one embodiment of the invention. [00022]
FIG. 13(b) is a Raman Spectroscopy graph of graphene oxide. [00024]
FIG. 14 is a Raman Spectroscopy graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00025]
FIG. 15 is a Raman Spectroscopy graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00026]
FIG. 16 is a Raman Spectroscopy graph of graphene prepared with Cand ida albica n s according to one embodiment of the invention. [00027]
FIG. 17 is a Raman Spectroscopy graph of graphene prepared with Streptococcus faecali s according to one embodiment of the invention. [00028]
FIG. 18 is a Raman Spectroscopy graph of graphene prepared with Staphylococcus a ureu s according to one embodiment of the invention.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of aerobically producing graphene from graphene oxide with a microorganism comprising: a) g rowing one or more microorganism cultures in suitable media under aerobic conditions; b) reducing concentration of the one or more cultures between approximately 10 2 CFU/mL to approximately 1 01 0 CFU/mL, wherein said reduced concentration comprises transferring a portion of the culture to a vessel; c) adding graphene oxide to the vessel under atmospheric conditions; d) removing the added graphene oxide from the vessel after a predetermined time, wherein said removed graphene oxide contains graphene; and e) washing the removed graphene oxide with a solution to recover the graphene, wherein the y ield of recovered graphene is greater than 50 % of the added graphene oxide; wherein the one or more microorganism cultures is selected from the group consisting of: Pseudomonas aeruginosa, Candida albicans, Streptococcus faecalis, Staphylococcus aureus, algae, cyanobacteria, and combinations thereof, wherein the microorganism reduces graphene oxide to graphene. Currently amended
The method of claim 1, wherein the suitable media is nutrient broth. Original
The method of claim 1, wherein the portion is approximately 10 1. Original
The method of claim 1, wherein the graphene oxide is a film of graphene oxide at a concentration of approximately 30mg per 10 4 CFU/mL of the one or more cultures. Previously presented
The method of claim 1, wherein the aerobic conditions comprise a period of 24 hours, a temperature from approximately 25 ° C to approximately 40 ° C, absence of light or a shaker without any additional gas. Original
The method of claim 1, wherein the concentration of the one or more cultures is approximately 1 0 4 CFU/mL. Original
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than 90/of the added graphene oxide. Currently amended
The method o claim 1, where the solution [[is]] in step e) comprises saline. Currently amended
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added in the absence of light. Withdrawn
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added under vacuum pressure. Withdrawn
The method of claim 1, wherein the predetermined
The method of claim 1, wherein the predetermined time is approximately 2-20 days. Original time is approximately 20 days. Original
Canceled
Canceled
Canceled
Canceled
Materials described outside the worked examples.
graphene oxide
graphene
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
graphite basal reflection (002) | 26.56 deg | natural graphite flakes |
graphene oxide diffraction peak |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,138,126Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 shows an exemplary process for producing graphene from GO according to one embodiment of the invention. [00010] FIGs. 2(a)-(b) are scanning electron …
FIGS. 2-6. SEM images may be aggregated, thin, crumpled sheets closely associated with each other and forming a disordered solid as illustrated in in
FIG. 7(a) is an X-Ray diffraction ("XRD") graph of graphite. [00016]
FIG. 8 is an XRD graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00018]
FIG. 9 is an XRD graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00019]
FIG. 10 is an XRD graph of graphene prepared with Can d ida a lb ica ns according to one embodiment of the invention. [00020]
FIG. 11 is an XRD graph of graphene prepared with Streptococcus fiaecalis according to one embodiment of the invention. [00021]
FIG. 12 is an XRD graph of graphene prepared with Staphylococcus aureus according to one embodiment of the invention. [00022]
FIG. 13(b) is a Raman Spectroscopy graph of graphene oxide. [00024]
FIG. 14 is a Raman Spectroscopy graph of graphene prepared with Escherichia co l i according to one embodiment of the invention. [00025]
FIG. 15 is a Raman Spectroscopy graph of graphene prepared with Pseudomonas aeruginosa according to one embodiment of the invention. [00026]
FIG. 16 is a Raman Spectroscopy graph of graphene prepared with Cand ida albica n s according to one embodiment of the invention. [00027]
FIG. 17 is a Raman Spectroscopy graph of graphene prepared with Streptococcus faecali s according to one embodiment of the invention. [00028]
FIG. 18 is a Raman Spectroscopy graph of graphene prepared with Staphylococcus a ureu s according to one embodiment of the invention.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of aerobically producing graphene from graphene oxide with a microorganism comprising: a) g rowing one or more microorganism cultures in suitable media under aerobic conditions; b) reducing concentration of the one or more cultures between approximately 10 2 CFU/mL to approximately 1 01 0 CFU/mL, wherein said reduced concentration comprises transferring a portion of the culture to a vessel; c) adding graphene oxide to the vessel under atmospheric conditions; d) removing the added graphene oxide from the vessel after a predetermined time, wherein said removed graphene oxide contains graphene; and e) washing the removed graphene oxide with a solution to recover the graphene, wherein the y ield of recovered graphene is greater than 50 % of the added graphene oxide; wherein the one or more microorganism cultures is selected from the group consisting of: Pseudomonas aeruginosa, Candida albicans, Streptococcus faecalis, Staphylococcus aureus, algae, cyanobacteria, and combinations thereof, wherein the microorganism reduces graphene oxide to graphene. Currently amended
The method of claim 1, wherein the suitable media is nutrient broth. Original
The method of claim 1, wherein the portion is approximately 10 1. Original
The method of claim 1, wherein the graphene oxide is a film of graphene oxide at a concentration of approximately 30mg per 10 4 CFU/mL of the one or more cultures. Previously presented
The method of claim 1, wherein the aerobic conditions comprise a period of 24 hours, a temperature from approximately 25 ° C to approximately 40 ° C, absence of light or a shaker without any additional gas. Original
The method of claim 1, wherein the concentration of the one or more cultures is approximately 1 0 4 CFU/mL. Original
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than of the added graphene oxide. Currently amended
The method of claim 1, wherein the yield of [[the]] recovered graphene is greater than 90/of the added graphene oxide. Currently amended
The method o claim 1, where the solution [[is]] in step e) comprises saline. Currently amended
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added in the absence of light. Withdrawn
(Withdrawn-Previously presented) The method of claim 1, wherein the graphene oxide is added under vacuum pressure. Withdrawn
The method of claim 1, wherein the predetermined
The method of claim 1, wherein the predetermined time is approximately 2-20 days. Original time is approximately 20 days. Original
Canceled
Canceled
Canceled
Canceled
Materials described outside the worked examples.
graphene oxide
graphene
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
graphite basal reflection (002) | 26.56 deg | natural graphite flakes |
graphene oxide diffraction peak |
Related documents with shared materials, methods, properties, or citations.
graphene oxide film
natural graphite flakes
nutrient broth
| 10.82 deg |
graphene oxide |
hexagonal graphite characteristic peak | 25.92 deg | graphene |
graphene yield from microbial reduction of GO | 95 % | graphene |
Temperature | 28–40 °C | — |
Duration | 8–10 hours | — |
Thickness | 100–3500 cm | — |
Temperature | 25–40 °C | — |
graphene oxide film
natural graphite flakes
nutrient broth
| 10.82 deg |
graphene oxide |
hexagonal graphite characteristic peak | 25.92 deg | graphene |
graphene yield from microbial reduction of GO | 95 % | graphene |
Temperature | 28–40 °C | — |
Duration | 8–10 hours | — |
Thickness | 100–3500 cm | — |
Temperature | 25–40 °C | — |
graphene oxide film
natural graphite flakes
nutrient broth
| 10.82 deg |
graphene oxide |
hexagonal graphite characteristic peak | 25.92 deg | graphene |
graphene yield from microbial reduction of GO | 95 % | graphene |
Temperature | 28–40 °C | — |
Duration | 8–10 hours | — |
Thickness | 100–3500 cm | — |
Temperature | 25–40 °C | — |
graphene oxide film
natural graphite flakes
nutrient broth
| 10.82 deg |
graphene oxide |
hexagonal graphite characteristic peak | 25.92 deg | graphene |
graphene yield from microbial reduction of GO | 95 % | graphene |
Temperature | 28–40 °C | — |
Duration | 8–10 hours | — |
Thickness | 100–3500 cm | — |
Temperature | 25–40 °C | — |