Tethering of Cofactors on Graphene-like Materials

and ZW.

Patent

Atlas literature

Patent

US 9,742,011

Tethering of Cofactors on Graphene-like Materials

Plamen B. Atanassov, Claudia W. Narvaez Villarrubia, Sergio Omar Garcia

STC.UNM·US

Drawings

Patent drawings and their descriptions. Click a drawing to enlarge it.

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FIG. 21

Claims

Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.

1 independent · 16 dependent

1
IndependentNAD(P)+/NAD(P)H cofactormulti-walled carbon nanotubessingle walled carbon nanotubesgraphenerGOgraphene-based or graphene-containing substrates
A complex formed from a NAD/(P)+/NAD(P)H cofactor tethered to a graphene-like material selected from the group consisting of multi-walled carbon nanotubes, single walled carbon nanotubes, graphene, rGO, and other graphene-based or graphene-containing substrates, wherein the cofactor is attached, via a covalent bond, to a pyrene butanoic acid succinimidyl ester tethering molecule which is attached to the graphene-like material via 2t-2 stacking.
1. 2
  Dependent← claim 1pyrene butanoic acid succinimidyl ester
  The complex of claim 1 wherein the tethering molecule comprises a polycyclic aromatic or polyarene foot and an ester moiety-containing alkyl-chain. canceled
2. 5
  Dependent← claim 1pyrene butanoic acid succinimidyl ester
  The complex of claim 1 wherein the tethering molecule is pyrene butanoic acid succinimidyl ester (PBSE). canceled
3. 7
  Dependent← claim 1multi-walled carbon nanotubes
  The complex of claim 1 wherein the graphene-like material is a multi-walled carbon nanotube (MWCNT).
4. 9
  Dependent← claim 1
  The complex of claim 1 wherein the cofactor is a dynamic cofactor. canceled
5. 10
  Dependent← claim 1, claim 9NAD(P)+/NAD(P)H cofactormulti-walled carbon nanotubessingle walled carbon nanotubesgraphenerGOgraphene-based or graphene-containing substrates
  The complex of claim 9 wherein the dynamic cofactor is NAD(P) +/NAD(P)H. 11. The complex of claim 1 further comprising a protein which requires the presence of the cofactor for biological function tethered to the graphene-like material. 12. The complex of claim 11 wherein the protein is an enzyme. 13. The complex of claim 11 wherein the enzyme protein is a biocatalyst. 14. A method for forming a biological complex attaching a cofactor to a graphene-like material comprising: 4 covalently attaching the a NAD(P)+/NAD(P)H cofactor to a pyrene butanoic acid succinimidyl ester tethering enzyme and; attaching the tethering enzyme to the a graphene-like material selected from the group consisting of multi-walled carbon nanotubes, single walled carbon nanotubes, graphene, rGO, and other graphene-based or graphene-containing substrates via 7[-7t stacking. currently amended
6. 20
  Dependent← claim 1NAD(P)+/NAD(P)H cofactor
  The method of claim 1 4 wherein the cofactor is NAD(P) +/NAD(P)H. 5 canceled
15
Dependent← claim 14
The method of claim 14 wherein the tethering enzyme comprises a polycyclic aromatic or polyarene foot and an ester moiety-containing alkyl-chain. canceled
1. 16
  Dependent← claim 15
  The method of claim 15 wherein the polycyclic aromatic or polyarene foot is selected from the group consisting of: a fused benzene structure, pyrene, pyrelene, benzo(a)pyrene, benzo(ghi)pyrene, corene, corannulene, ovalene, other molecular-benzene-based structures, cyclic aromatic hydrocarbons, and graphene-like structures. canceled
17
Dependent← claim 14pyrene butanoic acid succinimidyl ester
The method of claim 14 wherein the tethering molecule is pyrene butanoic acid succinimidyl ester (PBSE). canceled
19
Dependent← claim 14multi-walled carbon nanotubes
The method of claim 14 wherein the graphene-like material is a multi-walled carbon nanotube (MWCNT). canceled
21
Dependent← claim 14NAD(P)+/NAD(P)H cofactor
The method of claim 14 further comprising tethering a protein that interacts with the NAD(P) +/NAD(P)H confactor to the graphene-like material. 22. The method of claim 21 wherein the protein is an enzyme. 23. The method of claim 21 wherein the protein is a biocatalyst. 6

Materials

Materials described outside the worked examples.

NAD(P)+/NAD(P)H cofactor

Cofactor

multi-walled carbon nanotubes

Graphene-Like Substrate

Why these are connected

Related documents with shared materials, methods, properties, or citations.

Tethering of Cofactors on Graphene-like Materials | Matter42 Literature